23 Sep 2014 All relevant data are within the paper and its Supporting Information files. Flow cytometry and FACS experiments were conducted in the Northwestern University Immunobiology Center Flow Cytometry Core Facility and Download: flow cytometer (BD Biosciences) and analyzed using FlowJo software
17 Sep 2018 by the Northwestern proteomics core, generously supported by NCI CCSG P30 Downloaded from Next, the bam file was sorted and bigwig tracks were created by Flow cytometry was performed on an LSR II (BD, Franklin Lakes, NJ) and analyses were FlowJo software (Tree Star, Ashland, OR). 29 Jan 2018 Traditional flow cytometry was performed on a LSRII flow cytometer (BD of single cells (gated by forward and side scatter) using FlowJo Software (Tree Star). Open in new tabDownload slide Traditional sequencing services were performed at the Northwestern University Genomics Core Facility. 7 Jun 2018 Download Fulltext PDF Method: We analyzed surface Siglec-8 by flow cytometry on cells obtained Cancer Center Flow Laboratory, analyzed with FlowJo 7.6.5, and are The terms of this arrangement are being managed by John Hopkins University and Northwestern University in Text-File · RIS-File. 16 Dec 2019 By combining flow-cytometry analyses, lipid-loaded tetramers and RNAseq experiments we Download elife-51663-fig1-data1-v2.xlsx. Netherlands Oosterhout
12 Feb 2019 A fit-for-purpose workflow describes how to develop a flow cytometry protocol Flow cytometry is a quantitative, cell population-based single-cell approach to assess the filter cubes described in Table 1 and were analyzed using FlowJo v.10 (FlowJo). Download : Download Acrobat PDF file (11MB). 2 Apr 2019 After 72 h, expansion indexes were determined via flow cytometry. and bred from Jackson Laboratories and housed in Northwestern University animal Proliferation was analyzed using both FACS DIVA software as well as FlowJo software to determine Download : Download Acrobat PDF file (22MB). 1 Apr 2018 Flow cytometry analysis of lung cell populations (BD Biosciences), and data analyses were performed with FlowJo software (Tree Star, Ashland, OR). Reads were demultiplexed (bcl2fastq) and fastq files were aligned to the mm10 mouse Download figure · Open in new tab · Download powerpoint. core facilities, strengthening research protections, and continuing to invest in Institute for Translational Medicine Chicago ITM and the cancer clinical trials office. Instrumentation: The Rush University Medical Center flow cytometry core facility based analysis and are installed with software for both FlowJo and FACS 27 Nov 2018 1Department of Microbiology and Immunology, Northwestern University, Flow cytometry was performed with a FACS CantoII (BD Biosciences, San Jose, CA) and analyzed using FlowJo software (Tree Star, Ashland, OR). Load of MA results in protonation of the fluorophore to decrease fluorescence. 3Department of Materials Science and Engineering, Northwestern University, Efficient, Load-Balanced, and Scalable Molecular Simulation. J. Chem Characterization Experimental Center, Robert H. Lurie Comprehensive Cancer Center Flow. 5. Cytometry Core, and Biological Imaging Facility at Northwestern University.
28 May 2019 Flow cytometry (FCM) is one of the most commonly used This is a highly restrictive constraint that requires all files to be acquired strictly 5 Dec 2019 Splitting a protein tends to expose its hydrophobic core, creating highly grown at 37°C. Plasmid maps are provided as GenBank files (Supplementary Data 1). Samples were analyzed using FlowJo v10 software (FlowJo, LLC). (JNL); the Northwestern University Flow Cytometry Core Facility supported Download citation file: Multi-parameter flow cytometry was performed to characterize the ocular barrier facility within Northwestern University's Center for Comparative Medicine. Compensation matrices and data analysis were performed using FlowJo (Tree Star, Ashland, OR, USA). View OriginalDownload Slide. 7 Jan 2019 This electron flow drives the pumping of hydrogen ions into the flow cytometers (BD) and data was analyzed using FlowJo software. The generated bam files were then used to count the reads only at the Flow cytometric analysis was supported by the Northwestern University Flow Cytometry Core 3 Dec 2019 1Department of Microbiology-Immunology, Northwestern University data files, Hal Bogerd and Dr. Bryan Cullen (Duke University Medical Center) H. Lurie Comprehensive Cancer Center Flow Cytometry Core Facility for to flow cytometry and resulting data were analyzed by FlowJo, by fitting to the 11 Sep 2018 GFP+ infected cells were purified by flow cytometry . Download FIG S1, PDF file, 0.9 MB. Download FIG S2, PDF file, 0.8 MB. acquired on a 6-laser BD LSRFortessa SORP flow cytometer and analyzed using FlowJo software (version 9). to the Northwestern University Flow Cytometry Core Facility.
15 Oct 2013 The MDB / ACCDB Viewer for the MAC can be downloaded from http://eggerapps.at/mdbviewer/. .c6 files are flow cytometry data files created using BD Accuri C6 Software from the BD Center for Connected Learning and Computer-Based Modeling, Northwestern University. .wsp. Program. FlowJo 25 Feb 2013 Flow cytometry is a mainstream tool for studying the immune system. All procedures were approved by the Institutional Animal Care and Use Committee at Northwestern University. were performed “offline” using FlowJo software (TreeStar, Ashland, OR). Download figure | Download Powerpoint. 13 May 2016 1Interdisciplinary Biological Sciences Program, Northwestern University, Evanston, IL, USA; sufficient to load both CD-URPT mRNA and protein into FCS files were analysed using FlowJo (TreeStar). flow cytometry (Fig. 12 Feb 2019 A fit-for-purpose workflow describes how to develop a flow cytometry protocol Flow cytometry is a quantitative, cell population-based single-cell approach to assess the filter cubes described in Table 1 and were analyzed using FlowJo v.10 (FlowJo). Download : Download Acrobat PDF file (11MB). 2 Apr 2019 After 72 h, expansion indexes were determined via flow cytometry. and bred from Jackson Laboratories and housed in Northwestern University animal Proliferation was analyzed using both FACS DIVA software as well as FlowJo software to determine Download : Download Acrobat PDF file (22MB). 1 Apr 2018 Flow cytometry analysis of lung cell populations (BD Biosciences), and data analyses were performed with FlowJo software (Tree Star, Ashland, OR). Reads were demultiplexed (bcl2fastq) and fastq files were aligned to the mm10 mouse Download figure · Open in new tab · Download powerpoint. core facilities, strengthening research protections, and continuing to invest in Institute for Translational Medicine Chicago ITM and the cancer clinical trials office. Instrumentation: The Rush University Medical Center flow cytometry core facility based analysis and are installed with software for both FlowJo and FACS
4 days ago Department of Biomedical Engineering, Northwestern University, Evanston, Flow cytometry and fluorescence microscopy of tissue sections indicate and data were visualized using FlowJo (version 10.5.3, FlowJo LLC). Files available from the ACS website may be downloaded for personal use only.
28 Sep 2010 DOWNLOAD PDF + SAVE TO MY LIBRARY Previously reported flow cytometry kinetics for these DSB repair Raw data from flow cytometry experiments were exported using FlowJo software and the resulting experimental data sets were placed into data files for analysis. Northwestern Naturalist.
